This resource is under construction. Please be patient, I promise it will be complete some day soon – Alex
- The Sterile Workspace
- Choosing Your Organism
- Knowing Your Organism
- Mixing Media and Pouring Plates
- Mixing Antibiotic Media
- Sterile Technique
- Mixing Buffers
- DNA101
- Purification of Genomic DNA
- Boil Purification of Genomic DNA
- Genomic DNA Extraction with CTAB
- Bead-Beater Genomic DNA Extraction
- Plasmids101
- Purification of Plasmid DNA (Miniprep aka. Ethanol Lysis)
- Plasmid Insertion
- Agarose Electrophoresis
- Restriction Enzyme Digest
- Phosphorylation
- Ligation
- Polymerase Chain Reaction (PCR)
- Primer Design
- Gradient PCR
- Flanking Restriction Site addition
- Site Directed Mutagenesis
- Gibson Cloning Protocol
- Golden Gate Cloning Protocol
- Confirmation of Successful Edits
- RNA101
- RNA Purification
- RT-PCR
- RT-qPCR
- GBlock Design
- Backbone Selection
- CRISPR/CAS9
- Proteins101
- Induction of Protein Expression
- Cell Lysis Protocols
- His-Tag Protein Purification
- SDS-PAGE
- ELISA
- Western Blot
- Southern Blot?
- Mass Spec Prep
- Cell Assays
- Antibody Production
- In Silica