The Graduates Guide to Synthetic Biology

“The trouble with having an open mind, of course, is that people will insist on coming along and trying to put things in it.” – Sir Terry Pratchett

Congratulations! You’ve done some or all of your graduate degree in Biology, which means you’re probably brimming with knowledge of peptide and nucleotide mechanics.
If you’re really lucky – you’ve also had extensive practical experience in the laboratory. If so, perhaps you should find your way across to the Experts page.
However, perhaps you are like myself and many others – you finished your degree with a disappointing lack of practical PRACTISE. You did the prac, you poured the gel and then you promptly forgot the ratio of agarose to TAE. Your restriction digest went perfectly… but already you wonder what the name of the buffer that went with EcoRI was.
Sound familiar? Well you’re in the right place my friend. Whether you’re just starting your honours project, or striking out as an independent researcher – this resource will be expressly designed to help YOU apply the knowledge of your degree where it matters most > in the lab!

Introduce each section, then lead into a universal protocol landing page, shared between other skill sets.

  • Pouring plates
  • Mixing antibiotic media
  • Mixing buffers
  • Purification of Genomic DNA
  • Purification of Plasmid DNA
  • Agarose Electrophoresis
  • Imaging and Dyes
  • Purification
  • Sequencing prep
  • Interpreting Sequencing
  • Synthesis
  • Restriction Digest
  • Ligation
  • Heat Shock + Recovery
  • Primer Design
  • Part Design
  • Functional Proteins
  • Proteins for Purification
  • Purification Methodology
  • Logic Gates
  • ETC