Buffer P1 (Resuspension Buffer)
AuthorAlexander KellyVideo Timestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:25 3. Safety & MSDS: 1:05 4. How to Mix: 2:28 5. Longevity: 5:55 6. Outro & Credits: 6:14
Final Buffer Concentration:
50 mM Tris-Cl
10 mM EDTA
100 ug/uL RNAse A
pH 8.0
For mixing 200mL of Buffer:
1
Dissolve 1.21g Tris base and 0.75g EDTA-2H20 in 150mL Autoclaved dH20
2
Adjust pH to 8.0 with HCl using a pH meter
3
Adjust volume to 200mL with dH2O
4
Filter Sterilise or Autoclave your buffer.
5
Add 20mg RNase A. Store at 4C if this step is performed.
See video for 100mL and 1L recipes, safety instructions and much, much more.
Ingredients
Video Timestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:25 3. Safety & MSDS: 1:05 4. How to Mix: 2:28 5. Longevity: 5:55 6. Outro & Credits: 6:14
Final Buffer Concentration:
50 mM Tris-Cl
10 mM EDTA
100 ug/uL RNAse A
pH 8.0
Directions
For mixing 200mL of Buffer:
1
Dissolve 1.21g Tris base and 0.75g EDTA-2H20 in 150mL Autoclaved dH20
2
Adjust pH to 8.0 with HCl using a pH meter
3
Adjust volume to 200mL with dH2O
4
Filter Sterilise or Autoclave your buffer.
5
Add 20mg RNase A. Store at 4C if this step is performed.
See video for 100mL and 1L recipes, safety instructions and much, much more.
