Boil Purification of Genomic DNA
Add 50 µl of TE buffer to a PCR tube or Microcentrifuge tube
Using sterile technique, pick up a colony from your plate and shake the loop around in the 50 µl of TE buffer to detach some bacteria.
Heat the sample to 95°C for 15 minutes in a heat block or thermal cycler.
Optional: Centrifuge at max speed for 5 minutes to pellet the cells. Most of the genomic DNA should remain within the liquid supernatant. Gently remove it and add it to another tube, using a pipette and careful not to disturb the pellet.
Add 1-2 µl of your sample to your PCR master mix.
Note: Do not add more, or the EDTA in the TE buffer will inhibit the polymerase activity
Mix 3-7 µl of your sample with 3 µl of 6 x purple loading dye.
Ingredients
Directions
Add 50 µl of TE buffer to a PCR tube or Microcentrifuge tube
Using sterile technique, pick up a colony from your plate and shake the loop around in the 50 µl of TE buffer to detach some bacteria.
Heat the sample to 95°C for 15 minutes in a heat block or thermal cycler.
Optional: Centrifuge at max speed for 5 minutes to pellet the cells. Most of the genomic DNA should remain within the liquid supernatant. Gently remove it and add it to another tube, using a pipette and careful not to disturb the pellet.
Add 1-2 µl of your sample to your PCR master mix.
Note: Do not add more, or the EDTA in the TE buffer will inhibit the polymerase activity
Mix 3-7 µl of your sample with 3 µl of 6 x purple loading dye.