Print Options:

Boil Purification of Genomic DNA

 50 µl per sample of TE Buffer
1

Add 50 µl of TE buffer to a PCR tube or Microcentrifuge tube

2

Using sterile technique, pick up a colony from your plate and shake the loop around in the 50 µl of TE buffer to detach some bacteria.

3

Heat the sample to 95°C for 15 minutes in a heat block or thermal cycler.

4

Optional: Centrifuge at max speed for 5 minutes to pellet the cells. Most of the genomic DNA should remain within the liquid supernatant. Gently remove it and add it to another tube, using a pipette and careful not to disturb the pellet.

Using the purified Genomic DNA for PCR:
5

Add 1-2 µl of your sample to your PCR master mix.
Note: Do not add more, or the EDTA in the TE buffer will inhibit the polymerase activity

Visualising the purified Genomic DNA on a gel:
6

Mix 3-7 µl of your sample with 3 µl of 6 x purple loading dye.