Buffer N3 (Neutralisation Buffer)
AuthorAlexander KellyTimestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:30 3. Safety & MSDS: 1:24 4. How to Mix: 4:04 5. Longevity: 6:46 6. Outro & Credits: 7:14
Final Buffer Concentration:
4.20 M Gu-Hcl
0.90 M CH3COOK
4.8
For Mixing 200mL of Buffer:
1
Dissolve 80.25g Gu-HCl in 100mL Autoclaved dH2O.
2
Add 17.67g potassium acetate (if mixing yourself: 0.18mol = 10.09g KOH dissolved in water + 10.809mL glacial acetic acid).
3
Adjust the pH to 4.8 with glacial acetic acid.
4
Make up to 200mL with autoclaved dH2O.
5
Don’t need to sterilise.
Ingredients
Timestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:30 3. Safety & MSDS: 1:24 4. How to Mix: 4:04 5. Longevity: 6:46 6. Outro & Credits: 7:14
Final Buffer Concentration:
4.20 M Gu-Hcl
0.90 M CH3COOK
4.8
Directions
For Mixing 200mL of Buffer:
1
Dissolve 80.25g Gu-HCl in 100mL Autoclaved dH2O.
2
Add 17.67g potassium acetate (if mixing yourself: 0.18mol = 10.09g KOH dissolved in water + 10.809mL glacial acetic acid).
3
Adjust the pH to 4.8 with glacial acetic acid.
4
Make up to 200mL with autoclaved dH2O.
5
Don’t need to sterilise.
