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Buffer P1 (Resuspension Buffer)

Video Timestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:25 3. Safety & MSDS: 1:05 4. How to Mix: 2:28 5. Longevity: 5:55 6. Outro & Credits: 6:14
Final Buffer Concentration:
 50 mM Tris-Cl
 10 mM EDTA
 100 ug/uL RNAse A
 pH 8.0
For mixing 200mL of Buffer:
1

Dissolve 1.21g Tris base and 0.75g EDTA-2H20 in 150mL Autoclaved dH20

2

Adjust pH to 8.0 with HCl using a pH meter

3

Adjust volume to 200mL with dH2O

4

Filter Sterilise or Autoclave your buffer.

5

Add 20mg RNase A. Store at 4C if this step is performed.

See video for 100mL and 1L recipes, safety instructions and much, much more.