Buffer P2 (Lysis Buffer)
AuthorAlexander KellyVideo Timestamps:
Final Buffer Concentration:
200 mM NaOH
1 % SDS
For Mixing 200mL of Buffer:
1
Add 1.60g of NaOH to 20mL dH2O
2
Add 2g of SDS and allow everything to dissolve
3
Make up to 200mL
No need to sterilise
Ingredients
Video Timestamps:
Final Buffer Concentration:
200 mM NaOH
1 % SDS
Directions
For Mixing 200mL of Buffer:
1
Add 1.60g of NaOH to 20mL dH2O
2
Add 2g of SDS and allow everything to dissolve
3
Make up to 200mL
No need to sterilise
