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Buffer P2 (Lysis Buffer)

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Final Buffer Concentration:
 200 mM NaOH
 1 % SDS
For Mixing 200mL of Buffer:
1

Add 1.60g of NaOH to 20mL dH2O

2

Add 2g of SDS and allow everything to dissolve

3

Make up to 200mL

No need to sterilise