Buffer PB (Wash Buffer 1)
AuthoradminTimestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:44 3. Safety & MSDS: 1:20 4. How to Mix: 2:52 5. Longevity: 4:49 6. Outro & Credits: 5:00
Final Buffer Concentration:
5 M Gu-HCl
30 % Isopropanol
7.5 (optional)
For Mixing 200mL of Buffer:
1
Dissolve 95.53g Gu-HCl in 100mL Autoclaved dH2O
2
Add 60 mL isopropanol
3
Optional: Balance pH to 7.5
4
Make up to 200mL with dH2O
5
No need to sterilise, never autoclave flammable materials.
Ingredients
Timestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:44 3. Safety & MSDS: 1:20 4. How to Mix: 2:52 5. Longevity: 4:49 6. Outro & Credits: 5:00
Final Buffer Concentration:
5 M Gu-HCl
30 % Isopropanol
7.5 (optional)
Directions
For Mixing 200mL of Buffer:
1
Dissolve 95.53g Gu-HCl in 100mL Autoclaved dH2O
2
Add 60 mL isopropanol
3
Optional: Balance pH to 7.5
4
Make up to 200mL with dH2O
5
No need to sterilise, never autoclave flammable materials.
