Buffer PB (Wash Buffer 1)

Buffer PB (Wash Buffer 1)

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Timestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:44 3. Safety & MSDS: 1:20 4. How to Mix: 2:52 5. Longevity: 4:49 6. Outro & Credits: 5:00
Final Buffer Concentration:
 5 M Gu-HCl
 30 % Isopropanol
 7.5 (optional)

For Mixing 200mL of Buffer:
1

Dissolve 95.53g Gu-HCl in 100mL Autoclaved dH2O

2

Add 60 mL isopropanol

3

Optional: Balance pH to 7.5

4

Make up to 200mL with dH2O

5

No need to sterilise, never autoclave flammable materials.

 

Ingredients

Timestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:44 3. Safety & MSDS: 1:20 4. How to Mix: 2:52 5. Longevity: 4:49 6. Outro & Credits: 5:00
Final Buffer Concentration:
 5 M Gu-HCl
 30 % Isopropanol
 7.5 (optional)

Directions

For Mixing 200mL of Buffer:
1

Dissolve 95.53g Gu-HCl in 100mL Autoclaved dH2O

2

Add 60 mL isopropanol

3

Optional: Balance pH to 7.5

4

Make up to 200mL with dH2O

5

No need to sterilise, never autoclave flammable materials.

Buffer PB (Wash Buffer 1)
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