Buffer PB (Wash Buffer 1)

Timestamps: 1. Intro & Credits: 0:00 2. Role of Buffer: 0:44 3. Safety & MSDS: 1:20 4. How to Mix: 2:52 5. Longevity: 4:49 6. Outro & Credits: 5:00

Final Buffer Concentration:

5 M Gu-HCl

30 % Isopropanol

7.5 (optional)

For Mixing 200mL of Buffer:

Dissolve 95.53g Gu-HCl in 100mL Autoclaved dH2O

Add 60 mL isopropanol

Optional: Balance pH to 7.5

Make up to 200mL with dH2O

No need to sterilise, never autoclave flammable materials.