Dissolve 242.28mg Tris Base in 100mL Autoclaved dH20.
Note: Some EB recipes use 5mM Tris-Cl, so you can choose to add instead 121.14g. I have not noticed a difference.
Add 58.448mg EDTA (Only if you're making TE - do not add if you wish to make EB)
Balance pH to 8.0
Filter sterilise or autoclave. Add 15mL to a falcon tube for immediate use and refrigerate the rest.
Just autoclave water (MilliQ or RO) in a bunch of small bottles (keep those lids loose!). You can open a new bottle each day to ensure you're always working with the freshest water for all your experiments.