Measure out 10g of Tryptone and add it to your bottle.
Note: Peptone and Tryptone are interchangeable but will have minor influences on your experiment. Feel free to use one in place of the other, but don't go swapping mid experiment.

Measure out 5g of Yeast Extract and add it to your bottle.

Measure out 10g of NaCl and add it to your bottle. (LB-Miller)
Note: If you plan to use everyday table salt, double check to ensure it doesn't also contain anti-caking agent. This will inhibit bacterial growth.
Alternative LB Recipes: 5g NaCl (LB-Lennox), 0.5g NaCl (LB-Luria)

Add MilliQ or RO water to make volume up to 1L.
Note: It is possible to make LB media with tap water, but keep in mind that the mineral content will start to interfere with your experiments soon. If you don't have access to a water purification system - try hunt down a cheap purification system before it becomes a problem.

Tighten the lid of your bottle, then loosen by half a turn.

Sterilise 20 minutes/liquid cycle in an autoclave. Or use one of our cheaper sterilisation protocols.