Skip to content
BioFoundry
  • Home
  • Protocols
    • The Graduates Guide to Synthetic Biology
    • Protocols
    • Tools & Equipment
  • BioFoundry Courses
  • Projects
    • Open Insulin
    • AgriPhage (Superbugs)
    • Lab-Grown Meat
    • Rumen NeoGenesis
    • Direct Neural Interface
    • Synthetic Palm Oil
    • OpenAgar
  • Community
    • Biofoundry Facebook
    • BioHackSyd Facebook Group
    • YouTube
    • Twitter
    • Instagram
    • Twitch Channel
  • Twitch Stream
  • Getting here
  • Store
  • About
    • Membership Registration
    • Contact Us
    • What is BioHacking?
    • OGTR + AusBioHack!
  • Login

Miniprep Buffer Mixing

The plasmid miniprep (aka. Ethanol Lysis) is the premier method of plasmid purification and will be your bread and butter for working with most microorganisms. You might even want to try purifying larger volumes of plasmid DNA, via a midiprep or maxiprep.

The key difference between these processes is the silica column. I’m going to make a guide on these, which will be here one day – but in the meantime, the important point to understand is that you can purchase generic silica columns for ~0.50¢/each without having to shill out for a full miniprep kit. Keep in mind that columns can be manufactured to capture different sizes of DNA fragment and choosing the wrong type of column will impact your yield.

Once you’ve got the columns, you’re going to need buffers. Openwetware.org have been our primary source for the buffer recipes featured in our guides, but we also could not have succeeded without the mentoring of Dr. Nicholas Coleman.

Click through to the following pages for a demonstration video, protocols and additional resources for mixing each of the miniprep buffers:

  1. Buffer P1 (Resuspension Buffer)
  2. Buffer P2 (Lysis Buffer)
  3. Buffer N3 (Neutralisation Buffer)
  4. Buffer PB (Wash Buffer 1)
  5. Buffer PE (Wash Buffer 2)
  6. Buffer TE, Buffer EB and Water (Elution buffers)

Operating Hours

Monday Through Friday:
– 9am-2pm
Weekends:
– by appointment / events

Safety & Legal

Terms of Service
Our WHS policy
OGTR & PC1 Stuff
IBC / SWP / RA
Lab entry requirements

Register Now

Student and Pro memberships available

or support us on Patreon

© 2020 BioFoundry Ltd. All rights reserved.
Theme by Colorlib Powered by WordPress